Maturation pathway for Novikoff ascites hepatoma 5.8 S ribosomal ribonucleic acid. Evidence for its presence in 32 S nuclear ribonucleic acid.

Structural analyses of mammalian ribosomal ribonucleic acid and its precursors. Nucleotide sequence of ribosomal 5.8 S ribonucleic acid.

The nucleotide sequence of ribosomal 5.8 S RNA (also known as 7 S or 5.5 S rRNA) from Novikoff hepatoma ascites cells has been determined to be (see article). Estimations of the secondary structure based upon maximized base pairing and the fragments of partial ribonuclease digestion indicate that there may be five base-paired regions in the molecule, three forming a folding of the termini and t...

Inhibition of ribosomal ribonucleic acid maturation in Novikoff hepatoma cells by 5-fluorouracil and 5-fluorouridine.

5-Fluorouracil, at lop4 M concentrations, inhibited ribosomal RNA maturation only slightly in Novikoff hepatoma cells growing in suspension culture. On a molar basis, 5-fluorouridine was a much more potent inhibitor of the maturation process. The difference between the effectiveness of 5-fluorouracil and 5-fluorouridine is probably a result of the greater transport and incorporation of the latt...

Indirect evidence for the presence of Ribonucleic acid in vesicular stomatitis Virions

Quantitative determination of the ribosomal ribonucleic acid content of liver and Novikoff hepatoma from fed and from fasted rats.

Although never demonstrated unequivocally, it has been generally accepted that the largest fraction of the ribonucleic acid of mammalian cells is ribosomal. However, it has been reported recently that about 65% of rat liver RNA has a nonribosomal sedimentation coefficient in the range of 4 S. To resolve the question of the ribosomal RNA content of rat liver and to examine the validity of the as...

Primary sequence of U-1 nuclear ribonucleic acid of Novikoff hepatoma ascites cells.

The U-l RNA is one of the low molecular weight nuclear RNAs of Novikoff hepatoma ascites cells that is specifically localized to the extranucleolar portion of the nucleus. After the RNA was purified by polyacrylamide gel electrophoresis, it was subjected to various enzymatic digestion procedures for determination of its primary nucleotide sequence. The U-l RNA contains 171 nucleotides. Its 5’ e...